纳米银抗菌活性和细胞毒性评价

纳米银抗菌活性和细胞毒性评价

摘要：以大肠杆菌和金黄色葡萄球菌做模型检测抗菌活性,选择L-929细胞通过MTT法评价毒性.结果证明纳米银具有显著的抗菌活性且呈剂量依赖性;不论在液体培养基还是固体培养基,30×10浓度产生的抗菌作用大于50%,但有潜在的细胞毒性;即使在3×10浓度下.细胞毒性达到2级,有26%,～50%的细胞生长受到抑制.Abstract：E. coil and S. aureus were used as model for testing antimicrobial activity, and L-929 cell line was selected for toxicity evaluation by cellular mitochondrial function(MTT assay). The results show that silver nanoparticles(Ag NPs)can significantly in-crease antibacterial activity with the increasing of concentration of the silver nanoparticles. The lowest effective concentration of silver nanoparticles(30 X 10)which leads to more than 50% of inhibition in liquid medium and agar plate on bacterias is still satis-factory but will produce potential cytotoxicity. Even at 3 x 10 concentration of silver nanoparticles the cytotoxicity still exists with 2grade which represents 26%-50% of cell growth inhibited, and 30×10 displays similar impact. 作者： 李新平[1]李胜利[2]张淼涛[1]张文龙[1]李创宏[1] Author： Li Xinping[1]  Li Shengli[2]  Zhang Miaotao[1]  Zhang Wenlong[1]  Li Chuanghong[1] 作者单位： 西北农林科技大学,陕西,杨凌,712100中国农业大学动物营养国家重点实验室,北京,100083 期 刊： 稀有金属材料与工程   ISTICEISCIPKU Journal： RARE METAL MATERIALS AND ENGINEERING 年，卷(期)： 2011, 40(2) 分类号： Q2 关键词： 纳米银    大肠杆菌    金黄色葡萄球菌    抗菌活性    细胞毒性    Keywords： silver nanoparticles    S. aureus    E. coli    antibacterial activity    cytotoxicity    机标分类号： O6 P61 机标关键词： 纳米银    抗菌活性    细胞毒性评价    Ag Nanoparticles    silver nanoparticles    antimicrobial activity    antibacterial activity    toxicity evaluation    金黄色葡萄球菌    cell growth    液体培养基    剂量依赖性    固体培养基    L-929细胞    effective    cell line    细胞生长    浓度    模型检测    抗菌作用 基金项目： National Natural Science Foundation of China